Human PTP rho/PTPRT Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3697

R&D Systems, part of Bio-Techne
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AF3697
AF3697-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all PTP rho/PTPRT Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PTPρ/PTPRT
Gln26-Lys725
Accession # O14522

Specificity

Detects human PTPρ/PTPRT in Western blots. In Western blots, no cross-reactivity with recombinant human LAR, PTPRM, K, S, G or DEP1/CD148 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PTP rho/PTPRT Antibody

Detection of Human PTP?/PTPRT antibody by Western Blot.

Detection of Human PTPρ/PTPRT by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTP rho/PTPRT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3697) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTP rho/PTPRT at approximately 195 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human PTP rho/PTPRT Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line

Reviewed Applications

Read 3 reviews rated 4.3 using AF3697 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP rho/PTPRT

Protein Tyrosine Phosphatase, Receptor-type T (PTPRT), also known as RPTPrho, dephosphorylates tyrosine residues in proteins. PTPRT is present in many tissues, including brain, heart, lung, and colon. Genetic screening of colon cancers indicates that the PTPRT gene has a high incidence of missense mutations that reduce phosphatase activity in the encoded protein. Overexpression of intact PTPRT in cancer cell lines inhibits cell proliferation, suggesting that the phosphatase is a tumor suppressor.

Long Name

Protein Tyrosine Phosphatase Receptor-type T

Alternate Names

PTPRT, RPTP-rho

Entrez Gene IDs

11122 (Human); 19281 (Mouse); 362263 (Rat)

Gene Symbol

PTPRT

UniProt

O14522

Additional PTP rho/PTPRT Products

Product Documents for Human PTP rho/PTPRT Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PTP rho/PTPRT Antibody

For research use only

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