Human PTP sigma/PTPRS Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3430
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PTP sigma/PTPRS
Glu30-Gly1260
Accession # NP_570924
Glu30-Gly1260
Accession # NP_570924
Specificity
Detects the endogenous cleaved extracellular domain of human PTP sigma/PTPRS in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human LAR, PTPR beta, PTPR delta, PTPRµ, or PTPR kappa is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PTP sigma/PTPRS Antibody
Detection of Human PTP sigma/PTPRS by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human PTP sigma/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human PTPR mu, PTPR kappa, LAR, PTPR sigma, PTPR delta, and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PTP sigma/PTPRS in A431 Cell Line.
PTPs/PTPRS was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human PTPs/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PTP sigma/PTPRS Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line
Sample: SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTP sigma/PTPRS
Protein Tyrosine Phoshatase Receptor-type sigma (PTPR sigma), also known as PTP sigma, is an integral membrane protein with a non-glycosylated molecular weight of 214 kilodaltons. The extracellular domain, which was used as immunogen, is cleaved near the cell membrane by an endoprotease to generate a glycated 140 kDa protein. Significant levels of this protein are found in the brain and in endocrine tissues such as the pituitary gland and pancreas. Knockout mice deficient in PTPR sigma have severe neurological deficits and most die shortly after birth.
Long Name
Protein Tyrosine Phosphatase Receptor-type Sigma
Alternate Names
PTPR sigma, PTPRS
Gene Symbol
PTPRS
UniProt
Additional PTP sigma/PTPRS Products
Product Documents for Human PTP sigma/PTPRS Antibody
Product Specific Notices for Human PTP sigma/PTPRS Antibody
For research use only
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