Human PUM2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7415

R&D Systems, part of Bio-Techne
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MAB7415
MAB7415-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 740610

View all PUM2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PUM2
Pro103-Gly250
Accession # Q8TB72

Specificity

Detects human PUM2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PUM1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PUM2 Antibody

Detection of Human PUM2 antibody by Western Blot.

Detection of Human PUM2 by Western Blot.

Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PUM2 Monoclonal Antibody (Catalog # MAB7415) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for PUM2 at approximately 115-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PUM2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: KG‑1 human acute myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PUM2

The Pumilio proteins PUM1 and PUM2 are RNA binding proteins that are repressors of mRNA translation. PUM2 is present in embryonic stem cells and adult germ cells. Both positive and negative influences of PUM2 on proliferation have been reported. Human PUM2 is a 1066 amino acid (aa), 114 kDa (predicted) protein with 6 potential Ser/Thr phosphorylation sites (aa 82, 136, 178, 182, 184, 587, and 672), an acetylation site (aa 672), and a pumilio domain (aa 706-1048). Within aa 103-250, human PUM2 shares 95% aa sequence identity with mouse and rat PUM2. A potential 987 aa isoform lacks aa 574-652.

Long Name

Pumilio 2

Alternate Names

PUMH2, Pumilio-2, PUML2

Entrez Gene IDs

23369 (Human); 80913 (Mouse); 298874 (Rat)

Gene Symbol

PUM2

UniProt

Q8TB72

Additional PUM2 Products

Product Documents for Human PUM2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PUM2 Antibody

For research use only

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