Human PUM2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7415
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PUM2
Pro103-Gly250
Accession # Q8TB72
Pro103-Gly250
Accession # Q8TB72
Specificity
Detects human PUM2 in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human PUM1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PUM2 Antibody
Detection of Human PUM2 by Western Blot.
Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human PUM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7415) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PUM2 at approximately 115-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PUM2 Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: KG‑1 human acute myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line
Sample: KG‑1 human acute myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PUM2
PUM2 (Pumilio homolog 2/Pumilio-2) is a 121-125 kDa member of the PUF family of RNA-binding peoteins. It is expressed in a variety of cell types, including neurons, skeletal muscle cells, stem cells and germ cells, and plays a role in asymmetric cell division. PUM2 appears to bind to the 3' untranslated region of target mRNAs. By doing so, it causes a conformational change in the RNA, allowing for the subsequent binding of miRNAs and the silencing of protein translation. In germ cells, PUM2 is also reported to complex with NANOSI and SNAPIN, although its function in this complex is unclear. Human PUM2 is 1066 amino acids (aa) in length. It contains a SNAPIN interaction site (aa 1-260), one PUM-HD region (aa 706-1048) and eight consecutive pumilio repeats (aa 726-1022). There are six utilized Ser/Thr phosphorylation sites, and at least two isoform variants. One is 95-96 kDa in size and shows a deletion of aa 574-652, while another shows a deletion of two amino acids; Val829Ile830. Over aa 103-250, human PUM2 shares 95% aa sequence identity with mouse PUM2.
Long Name
Pumilio 2
Alternate Names
PUMH2, Pumilio-2, PUML2
Gene Symbol
PUM2
UniProt
Additional PUM2 Products
Product Documents for Human PUM2 Antibody
Product Specific Notices for Human PUM2 Antibody
For research use only
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