Human Rad50 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4996
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Rad50
Leu518-Leu881
Accession # Q92878
Leu518-Leu881
Accession # Q92878
Specificity
Detects human Rad50 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Rad50 Antibody
Detection of Human Rad50 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cells and MCF-7 human breast cancer cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Rad50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4996) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Rad50 at approximately ~150kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1Applications for Human Rad50 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cells and MCF‑7 human breast cancer cells
Sample: Jurkat human acute T cell leukemia cells and MCF‑7 human breast cancer cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rad50
Rad50 is a 150 kDa member of the Rad50 subfamily, SMC (structural maintenance of chromosomes) family of DNA-associated genes. It is ubiquitously expressed, and associates with MRE11 and NBS1 to form an MRN complex. This complex stabilizes ATM kinase, thus contributing to DNA repair, and also participates in the suppression of DNA rereplication in dividing cells. Human Rad50 is 1312 amino acids (aa) in length. It has an apparent ATP binding site (aa 36‑43) plus a coiled‑coil region (aa 228‑598) followed by a “zinc-hook” domain (aa 635‑734) that mediates homodimerization. There are multiple splice variants. An alternate start site exists at Met140, there is a single Lys substitution for aa 723‑1312, and three Lys substitute for aa 555‑1312. Over aa 518‑881, human Rad50 is 96% aa identical to mouse Rad50.
Alternate Names
DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2
Gene Symbol
RAD50
UniProt
Additional Rad50 Products
Product Documents for Human Rad50 Antibody
Product Specific Notices for Human Rad50 Antibody
For research use only
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