Human Rad51 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9948

R&D Systems, part of Bio-Techne
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MAB9948-100
MAB9948-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 523701

View all Rad51 Antibodies »

Product Summary for Human Rad51 Antibody

Immunogen

E. coli-derived recombinant human Rad51
Val174-Asp339
Accession # Q06609

Specificity

Detects human Rad51 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human Rad51 Antibody

Rad51 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

Rad51 in Human Breast Cancer Tissue.

Rad51 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Rad51 Monoclonal Antibody (Catalog # MAB9948) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Rad51 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rad51

Human Rad51 is a 339 amino acids, 37kDa protein that plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. RAD51 family members are homologous to the bacterial RecA and yeast Rad51 and are highly conserved in most eukaryotes, from yeast to humans. Different splice variants of the human RAD51 gene have been found.

Alternate Names

BRCC5, hRAD51, HsRAD51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A

Entrez Gene IDs

5888 (Human); 19361 (Mouse)

Gene Symbol

RAD51

UniProt

Q06609

Additional Rad51 Products

Product Documents for Human Rad51 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Rad51 Antibody

For research use only

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