Human/Rat alpha-Fetoprotein/AFP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1369
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Rat
Cited:
Human, N/A
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
ELISA Capture, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Chicken IgY
Product Specifications
Immunogen
Human umbilical cord serum-derived alpha-Fetoprotein
Specificity
Detects human alpha-Fetoprotein in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Scientific Data Images for Human/Rat alpha-Fetoprotein/AFP Antibody
Detection of Human and Rat alpha‑Fetoprotein/AFP by Western Blot.
Western blot shows lysates of human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Chicken Anti-Human/Rat alpha‑Fetoprotein/ AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for alpha‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells.
a-Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells using Chicken Anti-Human/Rat a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (red; Catalog # NL016) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
alpha‑Fetoprotein/AFP in Human Liver cancer
alpha‑Fetoprotein/AFP was detected in immersion fixed paraffin-embedded sections of human liver cancer using 15 µg/mL Chicken anti-Human/ Rat alpha‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Rat alpha-Fetoprotein/AFP Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer
Sample: Immersion fixed paraffin-embedded sections of human liver cancer
Knockout Validated
alpha‑Fetoprotein is specifically detected in HepG2 human hepatocellular carcinoma cell line but is not detectable in alpha‑Fetoprotein knockout HepG2 cell line.
Simple Western
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Western Blot
0.2 µg/mL
Sample: Human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line
Sample: Human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line
Reviewed Applications
Read 2 reviews rated 5 using AF1369 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified from egg yolks
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Fetoprotein/AFP
alpha‑Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors.
Additional alpha-Fetoprotein/AFP Products
Product Documents for Human/Rat alpha-Fetoprotein/AFP Antibody
Product Specific Notices for Human/Rat alpha-Fetoprotein/AFP Antibody
For research use only
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