Human/Rat FABP1/L-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB29643
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 965608
Product Specifications
Immunogen
E. coli-derived recombinant human FABP1/L-FABP
Met1-Ile127
Accession # P07148
Met1-Ile127
Accession # P07148
Specificity
Detects human and rat FABP1/L-FABP in direct ELISAs and Western blots. In sandwich immunoassays, it is specific for human FABP1/L-FABP.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Rat FABP1/L-FABP Antibody
Detection of Human and Rat FABP1/L‑FABP by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, Hep3B human hepatocellular carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29643) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat FABP1/L‑FABP by Simple WesternTM.
Simple Western lane view shows lysates of rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Rat FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29643) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Rat FABP1/L-FABP Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody for human FABP1/L-FABP when paired with Mouse Anti-Human FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29642).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Simple Western
10 µg/mL
Sample: Rat Liver Tissue
Sample: Rat Liver Tissue
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP1/L-FABP
FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.
Long Name
Fatty Acid-Binding Protein 1
Alternate Names
L-FABP, LFABP
Gene Symbol
FABP1
UniProt
Additional FABP1/L-FABP Products
Product Documents for Human/Rat FABP1/L-FABP Antibody
Product Specific Notices for Human/Rat FABP1/L-FABP Antibody
For research use only
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