Human/Rat GR/NR3C1 Antibody

Detection of Human and Rat GR/NR3C1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U-87 MG human glioblastoma/astrocytoma cell line, A549 human lung carcinoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Rat GR/NR3C1 Monoclonal Antibody (Catalog # MAB10144) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GR/NR3C1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GR/NR3C1 in HeLa Human Cell Line.

GR/NR3C1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Rat GR/NR3C1 Monoclonal Antibody (Catalog # MAB10144) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

GR/NR3C1 in Human Liver.

GR/NR3C1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human/Rat GR/NR3C1 Monoclonal Antibody (Catalog # MAB10144) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.