Human/Rat Myoglobin Antibody

Name: Human/Rat Myoglobin Antibody
Brand: R&D Systems
SKU: MAB9720

R&D Systems, part of Bio-Techne | Catalog # MAB9720

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 974102

View all Myoglobin Antibodies »

Product Summary for Human/Rat Myoglobin Antibody

Immunogen

Purified human cardiac Myoglobin antigen from human heart
Accession # P02144

Specificity

Detects human Myoglobin in direct ELISAs. Detects human and rat Myoglobin in Western blots. In Western blots, no detection of mouse myglobin was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human/Rat Myoglobin Antibody

Detection of Human and Rat Myoglobin by Western Blot.

Western blot shows lysates of human heart tissue, mouse heart tissue (negative control), rat heart tissue, and human liver tissue (negative control). PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Myoglobin at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Myoglobin in Human Heart.

Myoglobin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human and Rat Myoglobin by Simple Western^TM.

Simple Western lane view shows lysates of human heart tissue, rat heart tissue, and mouse heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Myoglobin at approximately 22 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Rat Myoglobin Antibody

Application

Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Simple Western

10 µg/mL
Sample: Human heart tissue and Rat heart tissue

Western Blot

0.05 µg/mL
Sample: Human Heart Tissue and Rat Heart Tissue

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myoglobin

Myoglobin is a 17KDa cytoplasmic oxygen-binding protein encoded by the MB gene and expressed in myocytes of the heart and skeletal muscle. Its name derives from its structural and functional similarity to hemoglobin, the oxygen binding protein found in red blood cells. Functions of myoglobin include oxygen storage and transport, as well as scavenging of NO and reactive oxygen species. Myoglobin also serves as a sensitive marker for muscle injury resulting from cardiac infarction. Myoglobin was the first protein to have its three-dimensional structure determined by X-ray crystallography.