Human/Rat Phospho-ATF2 (S490/S498) Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS064

R&D Systems, part of Bio-Techne
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PPS064
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all ATF2 Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S490/S498 of ATF2

Specificity

Human and rat ~74 kDa and ~54 kDa splice form of ATF2 phosphorylated at S490/S498 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Human/Rat Phospho-ATF2 (S490/S498) Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 dilution
Sample: (frozen sections)

Western Blot

1:1000 dilution
Sample: human melanoma cells incubated with varying doses of the radiomimetic drug NCS

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: ATF2

ATF2 (activating transcription factor 2; also CRE-BP1) is a 60 kDa member of the ATF subfamily, basic-region leucine zipper family of DNA binding proteins. It has at least two activities. First, it binds to an octameric palindromic CRE (cAMP response element) 5’-TGACGTCA-3’, and secondary, it shows histone acetyltransferase (HAT) activity towards histones H2B and H4. Its palindromic binding activity depends upon homodimerization, or heterodimerization with either c-Jun or CRE-Bpa. Human ATF2 is 505 amino acids (aa) in length, and contains a C2H2-type zinc finger segment (aa 25-49), a HAT region (aa 112-350), and a basic leucine zipper domain (aa 350-414). There are over a dozen alternate splice forms that, in their entirety, impact all segments of the molecule. ATF2 is phosphorylated at multiple sites, including S62/S90/S112/S340/S368/S490/S498 and T69/T71. The Thr phosphorylations increase transcriptional and HAT activity. Serine phosphorylations at S490/S498 by ATM contribute to DNA damage repair after ionizing radiation. Human ATF2 is greater that 95% aa identical to mouse ATF2 and 100% identical to rat ATF2.

References

  1. Maekawa, T. et al. (1989) EMBO J. 8:2023.
  2. Bailey, J. et al. (2002) J. Clin. Endocrinol. Metab. 87:1717.
  3. GenBank Accession # AAY17202-AAY17215.
  4. Morton, S. et al. (2004) FEBS Lett. 572:177.
  5. Kawasaki, H. et al. (2000) Nature 405:195.
  6. Bhoumik, A. et al. (2005) Mol. Cell 18:577.

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Entrez Gene IDs

1386 (Human); 11909 (Mouse); 81647 (Rat)

Gene Symbol

ATF2

Additional ATF2 Products

Product Documents for Human/Rat Phospho-ATF2 (S490/S498) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Rat Phospho-ATF2 (S490/S498) Antibody

For research use only

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