Human/Rat PKM1/2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7244
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 937801
Product Specifications
Immunogen
E. coli-derived recombinant human PKM2
Ser2-Pro531
Accession # P14618-1
Ser2-Pro531
Accession # P14618-1
Specificity
Detects human PKM2 in
direct ELISAs. Detects human and rat PKM1/2 in
Western blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Rat PKM1/2 Antibody
PKM2 in WI‑38 Human Cell Line.
PKM2 was detected in immersion fixed WI-38 human lung fibroblast cell line using Mouse Anti-Human/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB7244) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.PKM2 in Human Prostate.
PKM2 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB7244) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.PKM2 in Rat Skeletal Muscle.
PKM2 was detected in perfusion fixed frozen sections of rat skeletal muscle using Mouse Anti-Human/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB7244) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Human/Rat PKM1/2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed WI‑38 human lung fibroblast cell line
Sample: Immersion fixed WI‑38 human lung fibroblast cell line
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate and perfusion fixed frozen sections of rat skeletal muscle
Sample: Immersion fixed paraffin-embedded sections of human prostate and perfusion fixed frozen sections of rat skeletal muscle
Knockout Validated
PKM2 is specifically detected in HeLa human
cervical epithelial carcinoma parental cell line but is not detectable in PKM2 knockout HeLa cell line.
Simple Western
10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, U‑87 MG human glioblastoma/astrocytoma cell line, human skeletal muscle
Sample: A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, U‑87 MG human glioblastoma/astrocytoma cell line, human skeletal muscle
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKM2
aa 389‑433 of PKM2. This variant shows limited expression (striated muscle) and hyperbolic Michaelis-Menten kinetics. There are additional isoform variants of PKM2 that show either a deletion of aa 59‑132, or a 67 aa substitution for aa 1‑82. Over aa 434‑531, human PKM2 shares 95% aa sequence identity with mouse PKM2.
Long Name
Pyruvate Kinase, Muscle
Alternate Names
CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK
Gene Symbol
PKM
UniProt
Additional PKM2 Products
Product Documents for Human/Rat PKM1/2 Antibody
Product Specific Notices for Human/Rat PKM1/2 Antibody
For research use only
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