Human/Rat Rad17 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1926
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Rad17
Asn2-Arg240
Accession # O75943
Asn2-Arg240
Accession # O75943
Specificity
Detects human and rat Rad17 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Rat Rad17 Antibody
Detection of Human and Rat Rad17 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line and, NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Rad17 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Rat Rad17 Antibody
Application
Recommended Usage
Immunoprecipitation
3 µg/500 µg cell lysate
Sample: U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies
Sample: U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line and, NRK rat normal kidney cells
Sample: K562 human chronic myelogenous leukemia cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line and, NRK rat normal kidney cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rad17
The DNA damage response activates cell cycle checkpoints to allow time for DNA repair and ensure the fidelity of each cell cycle. The Rad genes were first identified in yeast as genes required for the DNA damage response. Human Rad17 bears homology to the replication factor C (RFC) proteins and interacts with the Rad9/Rad1/Hus1 complex in cells exposed to multiple types of genotoxic stress, including ionizing radiation (IR) and ultraviolet light (UV).
Alternate Names
CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, FLJ41520, hRad17, R24L, RAD1 (S. pombe) homolog, RAD1 homolog, RAD17 homolog (S. pombe), Rad17-like protein, RAD17Sp, Rad24, RF-C activator 1 homolog, RF-C/activator 1 homolog
Gene Symbol
RAD17
UniProt
Additional Rad17 Products
Product Documents for Human/Rat Rad17 Antibody
Product Specific Notices for Human/Rat Rad17 Antibody
For research use only
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