Human/Rat SOX10 Antibody

Detection of Mouse SOX10 by Immunocytochemistry/Immunofluorescence

JCV T-antigen is expressed in neural crest cells.Fluorescent images of bone marrow cells isolated from JCV T-antigen transgenic mice cultured in neural conditions show that these cells are positive for p75 (A,B), nestin (D,E) and SOX-10 (G,H), which are all markers of the neural crest. JCV T-antigen (J,K) was detected in the nucleus of p75 positive cells. Bone marrow cells cultured in mesenchymal media were negative for p75, nestin and SOX-10, and did not express JCV T-antigen (C, F, I, L). Cell nuclei were visualized with DAPI. (Scale bar: 100 µm). (M) Diagram illustrating expression of JCV T-antigen in bone marrow cells. T-antigen expression was associated with neural crest lineage cells of the bone marrow cultured in serum-free neural stem cell media supplemented with bFGF and EFG (left panel), while mesenchymal cells maintained in alpha-MEM in the presence of 20% serum were negative for T-antigen (right panel). (N) FACS analysis of neural crest cells and MSCs demonstrates positivity for JCV T-antigen in 99% of neural crest cells and lack of JCV T-antigen expression in MSCs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23805194), licensed under a CC-BY license. Not internally tested by R&D Systems.