Human/Rat Syntaxin 5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5687
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Syntaxin 5 isoform 2
Met55-Thr300
Accession # Q13190
Met55-Thr300
Accession # Q13190
Specificity
Detects human Syntaxin 5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) Syntaxin 7, rhSyntaxin 8, and rhSytaxin 12 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Rat Syntaxin 5 Antibody
Detection of Human Syntaxin 5 by Western Blot.
Western blot shows lysates of T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Syntaxin 5 at approximately 35 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Syntaxin 5 in SH-SY5Y Human Cell Line.
Syntaxin 5 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Syntaxin 5 in Human Brain.
Syntaxin 5 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Rat Syntaxin 5 Antibody
Application
Recommended Usage
Immunocytochemistry
1-15 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra) and immersion fixed frozen sections of rat brain
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra) and immersion fixed frozen sections of rat brain
Simple Western
25 µg/mL
Sample: T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line
Sample: T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line
Western Blot
0.5 µg/mL
Sample: T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line
Sample: T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syntaxin 5
Alternate Names
SED5, STX5
Gene Symbol
STX5
UniProt
Additional Syntaxin 5 Products
Product Documents for Human/Rat Syntaxin 5 Antibody
Product Specific Notices for Human/Rat Syntaxin 5 Antibody
For research use only
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