Human/Rat TC21/R-Ras2 Antibody

Name: Human/Rat TC21/R-Ras2 Antibody
Brand: R&D Systems
SKU: AF3605

R&D Systems, part of Bio-Techne | Catalog # AF3605

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all TC21/R-Ras2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TC21/R-Ras2
Met1-Phe204
Accession # P62070

Specificity

Detects human and rat TC21/R-Ras2 in Western blots. Does not detect recombinant human R-Ras or M-Ras (R‑Ras3).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Rat TC21/R-Ras2 Antibody

Detection of Human/Rat TC21/R-Ras2 by Western Blot.

Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat TC21/R-Ras2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for TC21/R-Ras2 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TC21/R‑Ras2 in Human Placenta.

TC21/R-Ras2 was detected in immersion fixed paraffin-embedded sections of normal human placenta using Goat Anti-Human/Rat TC21/R-Ras2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3605) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Rat TC21/R-Ras2 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

2 µg/mL
Sample: Rat-2 rat embryonic fibroblast cell line and CHP-100 human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TC21/R-Ras2

Teratocarcinoma oncogene 21 (TC21), also known as R-Ras2, is a small GTP-binding protein of the Ras superfamily of GTPases. TC21 comprises an R-Ras subfamily with fellow members R-Ras and M-Ras (R-Ras3). Overexpression and activating mutations of TC21 have been reported in several tumor tissues and cell lines.

Long Name

Teratocarcinoma Oncogene TC21

Alternate Names

RRAS2

Entrez Gene IDs

22800 (Human); 66922 (Mouse); 365355 (Rat)

Gene Symbol