Human Reg1A/B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB49371
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 431211
Product Specifications
Immunogen
E. coli-derived recombinant human Reg1A
Gln23-Asn166
Accession # P05451
Gln23-Asn166
Accession # P05451
Specificity
Detects human Reg1A in ELISAs. 100% cross-reactivity with recombinant human (rh) Reg1B in Western blot is observed. In direct ELISAs no
cross-reactivity with rhReg3A, recombinant mouse Reg1, or recombinant rat Reg1A
is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Reg1A/B Antibody
Reg1A in Human Pancreas.
Reg1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Reg1A Monoclonal Antibody (Catalog # MAB49371) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Reg1A/B Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Western Blot
1 µg/mL
Sample: Recombinant Human Reg1A
Sample: Recombinant Human Reg1A
Reviewed Applications
Read 2 reviews rated 4.5 using MAB49371 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Reg1A/B
Reg1A, also known as PTP, PSP, and lithostathine, is a member of the Reg family of secreted proteins with a C-type lectin domain. Due to variable glycosylation, pancreatic Reg1A exists as multiple species of 16‑18 kDa. Reg1A promotes the maintenance and growth of pancreatic islet beta-cells and intestinal villi. It is up‑regulated in pancreatitis and some carcinomas. Reg1A is an antigenic target in autoimmune diabetes. Human Reg1A shares 65%‑68% aa sequence identity with mouse and rat Reg1A.
Reg1B, also known as secretory pancreatic stone protein 2 and lithostathine 1 beta, is a type I subclass member of the Reg family, which comprises secreted proteins with a C-type lectin domain. Reg1B is highly related to Reg1A, a protein that is implicated in islet cell regeneration and diabetogenesis.Long Name
Regenerating Islet-derived 1A/B
UniProt
Additional Reg1A/B Products
Product Documents for Human Reg1A/B Antibody
Product Specific Notices for Human Reg1A/B Antibody
For research use only
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