Human Reg1A/B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4937
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Reg1A
Gln23-Asn166
Accession # P05451
Gln23-Asn166
Accession # P05451
Specificity
Detects human Reg1A in direct ELISAs. In direct ELISAs, approximately 80% cross-reactivity with recombinant human (rh) Reg1B is observed, approximately 20% cross-reactivity with recombinant mouse Reg1, and less than 5% cross-reactivity with rhReg3A and recombinant rat Reg1A is observed. 100% cross-reactivity with rhReg1B in Western blot is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Reg1A/B Antibody
Detection of Human Reg1A by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Reg1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4937) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Reg1A at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reg1A in Human Pancreas.
Reg1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Reg1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4937) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Reg1A/B Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Western Blot
1 µg/mL
Sample: Human pancreas tissue
Sample: Human pancreas tissue
Reviewed Applications
Read 1 review rated 4 using AF4937 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Reg1A/B
Reg1A (Regenerating gene IA/ alpha; also REG, ICRF, PSPS, PTP and Lithostathine-1 alpha) is a secreted, variably glycosylated 15-22 kDa type I member of the REG gene family of proteins. It is induced in proliferating cell types such as colonic epithelium, islet beta-cells, and multiple tumor types, and is constitutively expressed by pancreatic acinar cells. Thus, Reg1A is found both in blood (ng/mL quantities) and pancreatic secretions. It would appear that Reg1A plays a role in blocking apoptosis, and is upregulated by both IL-22 and IL-6. Human Reg1A is synthesized as a 166 amino acid (aa) precursor. It contains a 22 aa signal sequence plus a 144 aa mature region that is characterized by the presence of three intrachain disulfide bonds, one C-type lectin domain (aa 34-164), and a utilized N-terminal O-linked glycosylation site (Thr27). Variablility in the glycosylation pattern, plus proteolytic cleavage after Arg11 generates Reg1A isoforms in the 15-22 kDa range. There is one potential isoform variant that shows an alternative start site at Met9. Mature (aa 23-166) human Reg1A shares 76% and 69% aa sequence identity with mouse and rat Reg1A, respectively.
Reg1B, also known as secretory pancreatic stone protein 2 and lithostathine 1 beta, is a type I subclass member of the Reg family, which comprises secreted proteins with a C-type lectin domain. Reg1B is highly related to Reg1A, a protein that is implicated in islet cell regeneration and diabetogenesis.Long Name
Regenerating Islet-derived 1A/B
UniProt
Additional Reg1A/B Products
Product Documents for Human Reg1A/B Antibody
Product Specific Notices for Human Reg1A/B Antibody
For research use only
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