Human Reg3A Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5940

R&D Systems, part of Bio-Techne
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AF5940
AF5940-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Reg3A Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Reg3A
Glu27-Asp175
Accession # Q06141

Specificity

Detects human Reg3A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Reg3G, recombinant mouse (rm) Reg3A, rmReg3B and recombinant rat Reg2 is observed and less than 2% cross-reactivity with rhReg1A, rhReg1B, rmRegIV, rmReg2 and rmReg3D.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Reg3A Antibody

Detection of Human Reg3A antibody by Western Blot.

Detection of Human Reg3A by Western Blot.

Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Human Reg3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5940) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Reg3A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Reg3A Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human small intestine tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 3.3 using AF5940 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg3A

Reg3A (regenerating gene III alpha) is a secreted, 17 kDa, type III member of the Reg family, C-type lectin superfamily of proteins. It is expressed in intestine and exocrine pancreas, where it may have a protective effect against infection and TNF-alpha -induced stress. Mature mouse Reg3A is 149 amino acids (aa) in length (aa 27‑175). It is essentially a soluble C-type lectin domain (aa 40‑173). There are four type III genes ( alpha-delta) in the mouse Reg family. Reg3A has less that 60% aa identity to beta-delta. Mature mouse Reg3A is 87% and 61% aa identical to rat and human Reg3A, respectively.

Long Name

Regenerating Islet-derived IIIa

Alternate Names

Lithostathine 3, PAP2, REG3A

Entrez Gene IDs

5068 (Human); 19694 (Mouse); 171162 (Rat)

Gene Symbol

REG3A

UniProt

Q06141 (Human)

Additional Reg3A Products

Product Documents for Human Reg3A Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Reg3A Antibody

For research use only

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