Human Resistin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB13591

R&D Systems, part of Bio-Techne
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MAB13591-100
MAB13591-500
MAB13591-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Capture, ELISA Development, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 184305

View all Resistin Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Resistin

Specificity

Detects human Resistin in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse RELM alpha.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Resistin Antibody

Application
Recommended Usage

Human Resistin Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Resistin Biotinylated Antibody (Catalog # BAM1359)
  • Standard: Recombinant Human Resistin Protein, CF (Catalog # 1359-RN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB13591 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Resistin

Resistin (resistance-to-insulin), also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a 10 kDa member of a small family of secreted cysteine-rich peptide hormones. These molecules purportedly play some role in inflammation, glucose metabolism, and angiogenesis (1-4). Human Resistin precursor is 108 amino acids (aa) in length. It contains an 18 aa signal sequence plus a 90 aa mature region. The mature region shows an N-terminal alpha-helical tail (aa 23-44) and a C-terminal beta-sheet globular head (aa 47-108) (5-7). The Resistin molecule circulates as either a noncovalent trimer (minor form), or a disulfide-linked homohexamer (major form). Noncovalent trimers are generated when the alpha-helical segments self-associate to form a three-stranded coiled-coil structure. Covalent hexamers subsequently appear when the free Cys at position #26 is engaged by adjacent trimers. It is hypothesized that the hexamer is the inactive form of the molecule, and bioactivity is achieved at the target site by disulfide bond reduction (5). Although Resistin family molecules can noncovalently interact to form heterotrimers in vitro, there is no evidence to suggest this occurs in vivo with Resistin (8, 9). Mature human Resistin shares 56% and 54% aa identity with mouse and rat Resistin, respectively. Rat Resistin possesses an alternate start site at Met48; this Met is not found in the mouse molecule, however (10). Rodent resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium (6, 11, 12). Although the function of Resistin is unclear, it would seem to block insulin-stimulated uptake of glucose by adipocytes and promote glucose release by hepatocytes (6, 13, 14). As such, it has been proposed to participate in diet-induced insulin-sensitivity. Diets high in fat promote an increase in overall adipocyte size. Hypertrophic adipocytes are known to secrete TNF-alpha which acts locally to block ACRP30 production. Since ACRP30 is an insulin-sensitizer, a drop in ACRP30 availability leads to insulin-insensitivity, which drives increased insulin production (a compensatory mechanism). High insulin induces Resistin secretion which now antagonizes insulin action, prompting more insulin production and more Resistin secretion (15).

References

  1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
  2. Garrod, D.R. et al. (2002) Mol. Membrane Biol. 19:81.
  3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
  4. King, I.A. et al. (1993) Genomics 18:185.
  5. Theis, D.G. et al. (1993) Int. J. Dev. Biol. 37:101.
  6. King, I.A. et al. (1996) J. Invest. Dermatol. 107:531.
  7. Nuber, U.A. et al. (1996) Eur. J. Cell Biol. 71:1.
  8. Chidgey, M. et al. (2001) J. Cell Biol. 155:821.
  9. Khan, K. et al. (2006) Br. J. Cancer 95:1367.
  10. Hashimoto, T. et al. (1997) J. Invest. Dermatol. 109:127.
  11. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
  12. Descargues, P. et al. (2006) J. Invest. Dermatol. 126:1622.

Alternate Names

ADSF, FIZZ3, HXCP1, RETN

Entrez Gene IDs

56729 (Human); 57264 (Mouse)

Gene Symbol

RETN

Additional Resistin Products

Product Documents for Human Resistin Antibody

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Product Specific Notices for Human Resistin Antibody

For research use only

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