Human Rex-1/ZFP42 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3598
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 373722
Product Specifications
Immunogen
E. coli-derived recombinant human Rex-1
Ser2-Lys150
Accession # NP_777560
Ser2-Lys150
Accession # NP_777560
Specificity
Detects human Rex-1 in direct ELISAs and Western blots. Does not cross-react with recombinant human YY1.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Rex-1/ZFP42 Antibody
Rex-1 in NTera-2 Human Cell Line.
Rex-1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Human Rex-1 Monoclonal Antibody (Catalog # MAB3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Rex-1/ZFP42 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line
Western Blot
1 µg/mL
Sample: Recombinant Human Rex-1
Sample: Recombinant Human Rex-1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rex-1/ZFP42
Human Rex-1 (also Zfp-42) is a 35 kDa zinc finger family transcription factor that is highly expressed in human embryonic stem cells. It is one of several gene markers used to identify human stem cells. It is 310 amino acids in length and contains a zinc finger-binding domain between aa 214-307. Over this region, human and mouse show modest orthology at 47% aa identity.
Long Name
Zinc Finger Protein 42
Alternate Names
Rex1, ZFP42
Gene Symbol
ZFP42
UniProt
Additional Rex-1/ZFP42 Products
Product Documents for Human Rex-1/ZFP42 Antibody
Product Specific Notices for Human Rex-1/ZFP42 Antibody
For research use only
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