Human RFC1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6457
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RFC1
Met800-Ser1093
Accession # P35251
Met800-Ser1093
Accession # P35251
Specificity
Detects human RFC1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human RFC1 Antibody
Detection of Human RFC1 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human RFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RFC1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human RFC1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RFC1
RFC1 (Replication factor C subunit 1; also RFC140) is a 140‑150 kDa, ubiquitously expressed member of the activator 1 large subunit family of proteins. It is one of five RFC complex subunits that participate in the early stages of DNA replication. Following the initiation of DNA synthesis by pol alpha/primase, an RFC complex displaces pol alpha at the replication fork, and recruits a PCNA homotrimeric complex to this site. This interacts with pol delta to extend the newly‑formed DNA strand. Human RFC1 is 1148 amino acids (aa) in length. It contains a phosphorylated-DNA binding region (aa 366‑477), a PCNA binding domain (aa 478‑712), an ATPase site (aa 643‑772), and an NLS (aa 1120‑1124). Caspase-3 cleaves RFC-1 into 97, 73 and 65 kDa fragments during apoptosis. There is one potential alternative start site at Met117. Over aa 800‑1093, human RFC1 shares 90% aa identity with mouse RFC1.
Long Name
Replication Factor C 1
Alternate Names
MHCBFB, PO-GA, RECC1, RFC140
Gene Symbol
RFC1
UniProt
Additional RFC1 Products
Product Documents for Human RFC1 Antibody
Product Specific Notices for Human RFC1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...