Human ROBO2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3147
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Avian - Chicken
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO2
Ser22-Ala312, RPVA, Pro313-Pro859
Accession # Q9HCK4
Ser22-Ala312, RPVA, Pro313-Pro859
Accession # Q9HCK4
Specificity
Detects human ROBO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO3 and rhROBO4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human ROBO2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: NTera-2 human testicular embryonic carcinoma cell line differentiated with retinoic acid
Sample: NTera-2 human testicular embryonic carcinoma cell line differentiated with retinoic acid
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human neural progenitor cells differentiated by growth factor withdrawal
Sample: Immersion fixed human neural progenitor cells differentiated by growth factor withdrawal
Western Blot
0.1 µg/mL
Sample: Recombinant Human ROBO2 Fc Chimera (Catalog # 3147-RB)
Sample: Recombinant Human ROBO2 Fc Chimera (Catalog # 3147-RB)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ROBO2
Human ROBO2 is a 175 kDa member of the ROBO/roundabout receptor family and is a 1357 amino acid (aa) type I transmembrane (TM) protein. It contains an extracellular domain (ECD) comprised of five C2-type Ig-like domains and three fibronectin type III domains. ROBO receptors interact with SLIT proteins to regulate axon guidance. Similar to ROBO1, the first two Ig-like domains of ROBO2 are predicted to be important for SLIT binding. Alternate splice forms of ROBO2 exist. The ROBO2 ECD in human shares 98% aa identity with the ROBO2 ECD in mouse and canine.
Long Name
Roundabout Homolog 2
Alternate Names
SAX3
Gene Symbol
ROBO2
UniProt
Additional ROBO2 Products
Product Documents for Human ROBO2 Antibody
Product Specific Notices for Human ROBO2 Antibody
For research use only
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