Human ROR gamma/RORC/NR1F3 PerCP-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC6109C
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation = 482 and 564 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 600214
Product Specifications
Immunogen
E. coli-derived recombinant human ROR gamma/RORC/NR1F3
Met1-Gln100
Accession # P51449-2
Met1-Gln100
Accession # P51449-2
Specificity
Detects human and mouse ROR gamma/RORC/NR1F3 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human ROR gamma/RORC/NR1F3 PerCP-conjugated Antibody
Detection of ROR gamma/RORC/NR1F3 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA, Calcium Ionomycin, LPS, Recombinant Human IL-6 (Catalog # 206-IL) and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human ROR gamma/RORC/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6109C) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human ROR gamma/RORC/NR1F3 PerCP-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, Recombinant Human IL-6 (Catalog # 206-IL), and Recombinant Human IL‑23 (Catalog # 1290-IL) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, Recombinant Human IL-6 (Catalog # 206-IL), and Recombinant Human IL‑23 (Catalog # 1290-IL) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR gamma/RORC/NR1F3
ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑100 human RORC shares 96% identity with mouse ROR gamma.
Long Name
Retinoic Acid-Related Orphan Receptor gamma
Alternate Names
NR1F3, RORC, RZRG
Entrez Gene IDs
6097 (Human)
Gene Symbol
RORC
UniProt
Additional ROR gamma/RORC/NR1F3 Products
Product Documents for Human ROR gamma/RORC/NR1F3 PerCP-conjugated Antibody
Product Specific Notices for Human ROR gamma/RORC/NR1F3 PerCP-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...