Human S100A1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4476
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human S100A1
Met1-Ser94
Accession # P23297
Met1-Ser94
Accession # P23297
Specificity
Detects human S100A1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) S100B, rhS100P, and rhS100A2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human S100A1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human S100A1
Sample: Recombinant Human S100A1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A1
S100A1 (also S100 alpha) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is expressed by striated muscle, endothelium and chondrocytes. Intracellularly, S100A1 regulates cytosolic Ca levels, and is part of the Hsp70/Hsp90 mutlichaperone complex that protects against protein denaturation. Extracellularly, it exists as both a homodimer and heterodimer with S100B, S100A4 and S100P, may bind to RAGE, and blocks apoptosis. Human S100A1 is 94 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑48 and 50‑85) and a Ca-dependent protein interaction site (aa 89‑91). There may be one alternate start site 53 aa upstream of the standard start site. Full-length human S100A1 shares 93% and 98% aa identity with mouse and canine S100A1, respectively.
Long Name
S100 Calcium Binding Protein A1
Alternate Names
protein S100-A1, S100 alpha, S100 calcium binding protein A1, S100 calcium-binding protein A1S100, S-100 protein alpha chain, S-100 protein subunit alpha, S100 protein, alpha polypeptide, S100A, S100-alpha
Gene Symbol
S100A1
UniProt
Additional S100A1 Products
Product Documents for Human S100A1 Biotinylated Antibody
Product Specific Notices for Human S100A1 Biotinylated Antibody
For research use only
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