Human S100A8/S100A9 Heterodimer Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB45701

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB45701-100
MAB45701-SP
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, Immunohistochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1099F

View all S100A8/S100A9 Heterodimer Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100A8/S100A9 heterodimer
Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9)
Accession # P05109 (S100A8) and P06702 (S100A9)

Specificity

Detects human S100A8/S100A9 in direct ELISAs. In ELISAs, it detects recombinant human S100A8/S100A9 heterodimer but it does not detect recombinant human S100A8 or S100A9 monomers.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human S100A8/S100A9 Heterodimer Antibody

S100A8/S100A9 Heterodimer antibody in HL60 Human Cell Line by Immunocytochemistry (ICC).

S100A8/S100A9 Heterodimer in HL60 Human Cell Line.

S100A8/S100A9 Heterodimer was detected in immersion fixed HL60 human promyelocytic leukemia cell line using Rabbit Anti-Human S100A8/S100A9 Heterodimer Monoclonal Antibody (Catalog # MAB45701) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (punctate). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
S100A8/S100A9 Heterodimer antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P).

S100A8/S100A9 Heterodimer in Human Lung Cancer Tissue.

S100A8/S100A9 Heterodimer was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Rabbit Anti-Human S100A8/S100A9 Heterodimer Monoclonal Antibody (Catalog # MAB45701) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tumor cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
S100A8/S100A9 antibody in Human Metastatic Tonsil Cancer Tissue Using Dual RNAscope®ISH and IHC.

S100A8/S100A9 in Human Metastatic Tonsil Cancer Tissue Using Dual RNAscope®ISH and IHC.

Syndecan-1/CD138 mRNA was detected (top image) in formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil cancer tissue probed with ACD RNAScope®Probe (Catalog # 425271) and stained using ACD RNAscope®2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) was processed for immunohistochemistry using R&D Systems Rabbit Anti-Human S100A8/S100A9 Monoclonal Antibody (Catalog# MAB45701) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC003) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue).

Applications for Human S100A8/S100A9 Heterodimer Antibody

Application
Recommended Usage

Dual RNAscope ISH-IHC Compatible

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded tissue sections of human metastatic tonsil cancer tissue

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HL60 human promyelocytic leukemia cell line

Immunohistochemistry

10-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB45701 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A8/S100A9 Heterodimer

S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins (1, 2). They are up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (3-5). The 10 kDa human S100A8 and 14 kDa S100A9 each contain two EF-hand calcium binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively (6, 7). In the presence of calcium or zinc, S100A8 and S100A9 associate into non-covalent homodimers and 34-35 kDa heterodimers with each other (8-10). The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (11). The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation (3, 12). S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (4, 5, 9).

References

  1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
  2. Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893.
  3. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
  4. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
  5. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
  6. Odink, K. et al. (1987) Nature 330:80.
  7. Dorin, J.R. et al. (1987) Nature 326:614.
  8. Teigelkamp, S. et al. (1991) J. Biol. Chem. 266:13462.
  9. Ryckman, C. et al. (2003) J. Immunol. 170:3233.
  10. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
  11. Damo, S.M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:3841.
  12. Ryu, M-J. et al. (2012) J. Biol. Chem. 287:22948.

Long Name

S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9

Alternate Names

Calprotectin

Entrez Gene IDs

6279 (Human)

Gene Symbol

S100A8

UniProt

P05109 (S100A8) and P06702 (S100A9)

Additional S100A8/S100A9 Heterodimer Products

Product Documents for Human S100A8/S100A9 Heterodimer Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100A8/S100A9 Heterodimer Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

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