Human S100B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB18204

R&D Systems, part of Bio-Techne
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MAB18204-100
MAB18204-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1046211

View all S100B Antibodies »

Product Specifications

Immunogen

Human S100B synthetic peptide
Accession # P04271

Specificity

Detects human S100B in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human S100B Antibody

S100B in WM-115 Human Cell Line.

S100B was detected in immersion fixed WM-115 human malignant melanoma cell line (positive staining) and MCF-7 human breast cancer cell line (negative staining) using Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB18204) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human S100B Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed WM‑115 human malignant melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of Ca2+ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Long Name

S100 Calcium Binding Protein B

Alternate Names

beta (neural), NEF, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100beta

Entrez Gene IDs

6285 (Human)

Gene Symbol

S100B

UniProt

P04271

Additional S100B Products

Product Documents for Human S100B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100B Antibody

For research use only

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