Human S100B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1820
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 472806
Product Specifications
Immunogen
E. coli-derived recombinant human S100B
Met1-Glu92
Accession # P04271
Met1-Glu92
Accession # P04271
Specificity
Detects human S100B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) S100A1, 2, 6, 7, 10, 11, 13, 16, rmS100A4, 8, 9, or rhS100P.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human S100B Antibody
Detection of Human S100B by Western Blot.
Western blot shows lysate of human brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB1820) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100B at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human S100B by Simple WesternTM.
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for S100B at approximately 4 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB1820) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human S100B Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Human brain (cerebellum) tissue
Sample: Human brain (cerebellum) tissue
Western Blot
2 µg/mL
Sample: Human brain (cerebellum) tissue
Sample: Human brain (cerebellum) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100B
Long Name
S100 Calcium Binding Protein B
Alternate Names
beta (neural), NEF, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100beta
Entrez Gene IDs
6285 (Human)
Gene Symbol
S100B
UniProt
Additional S100B Products
Product Documents for Human S100B Antibody
Product Specific Notices for Human S100B Antibody
For research use only
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