Human S100P Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2957
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Knockout Validated, Western Blot
Cited:
ELISA Development, ELISA Development (Detection), Immunohistochemistry, Immunohistochemistry-Paraffin, In-Cell Western (ICW), Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 357517
Product Specifications
Immunogen
E. coli-derived recombinant human S100P
Met1-Lys95
Accession # P25815
Met1-Lys95
Accession # P25815
Specificity
Detects human S100P in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) S100A10 or rhS100B is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human S100P Antibody
Detection of Human S100P by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma parental cell line and S100P knockout HT-29 cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100P at approximately 10 kDa (as indicated) in the parental HT-29 cell line, but is not detectable in knockout HT-29 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100P in NTera-2 Human Cell Line.
S100P was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
S100P Specificity is Shown by Immunocytochemistry in Knockout Cell Line.
S100P was detected in immersion fixed HT-29 human colon adenocarcinoma cell line but is not detected in S100P knockout (KO) HT-29 Human Cell Line cell line using Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human S100P Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line
Knockout Validated
S100P is specifically detected in HT‑29 human colon adenocarcinoma parental cell line but is not detectable in S100P knockout HT‑29 cell line.
Western Blot
1 µg/mL
Sample: Detection of human S100P in HT-29 cell line and knockout HT-29 cell line
Sample: Detection of human S100P in HT-29 cell line and knockout HT-29 cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100P
Human S100P is a 22 kDa, homodimeric member of the S100 family of calcium-binding proteins. The S100 family currently has more than 20 members and belongs to the EF-hand superfamily of molecules. S100P is 95 aa in length and contains short, sequential modules. There is an N-terminal alpha-helix, a unique EF-hand motif, an alpha-helix, a linker region, an alpha-helix, a classic EF-hand motif and a C-terminal alpha-helix. The EF-hand motif binds calcium, which likely alters molecular conformation. The rearranged S100P now binds ligand with the linker region. Human S100P shares 47% aa sequence identity with rat S100P.
Long Name
S100 Calcium Binding Protein P
Alternate Names
MIG9, migration-inducing gene 9, Protein S100-E, S100 calcium binding protein P, S100 calcium-binding protein Pprotein S100-P, S100E
Entrez Gene IDs
6286 (Human)
Gene Symbol
S100P
UniProt
Additional S100P Products
Product Documents for Human S100P Antibody
Product Specific Notices for Human S100P Antibody
For research use only
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