Human SALM2/LRFN1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5669
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 610712
Product Summary for Human SALM2/LRFN1 Antibody
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SALM2
Gln32-Gly534 (predicted)
Accession # Q9P244
Gln32-Gly534 (predicted)
Accession # Q9P244
Specificity
Detects human SALM2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SALM1, 3, or 4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SALM2/LRFN1 Antibody
SALM2 in Human Cerebellum.
SALM2 was detected in immersion fixed paraffin-embedded sections of human cerebellum using Human SALM2/LRFN1 Monoclonal Antibody (Catalog # MAB5669) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human SALM2/LRFN1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cerebellum
Sample: Immersion fixed paraffin-embedded sections of human cerebellum
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SALM2/LRFN1
SALM2 (synaptic adhesion-like molecule 2), also called LRFN1 (leucine-rich repeat and fibronectin type-III domain-containing protein 1) is a 105 kDa member of the LRFN family of type I transmembrane glycoproteins. The 740 amino acid (aa) mature human SALM2 contains a 505 aa extracellular domain that includes seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain. Mature human SALM2 shares 99% aa identity with mouse and rat SALM2. SALM2 co-localizes with both pre- and post-synaptic proteins at excitatory synapses in mature neurons and promotes neurite outgrowth.
Long Name
Synaptic Adhesion-like Molecule 2/Leucine-rich Repeat and Fibronectin Type III Domain Containing 1
Alternate Names
LRFN1
Gene Symbol
LRFN1
UniProt
Additional SALM2/LRFN1 Products
Product Documents for Human SALM2/LRFN1 Antibody
Product Specific Notices for Human SALM2/LRFN1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...