Human Serpin A1/ alpha1-Antitrypsin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1268
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A1/ alpha1‑Antitrypsin
Glu25-Lys418
Accession # P01009
Glu25-Lys418
Accession # P01009
Specificity
Detects human Serpin A1/ alpha1‑Antitrypsin in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Serpin A1/ alpha1-Antitrypsin Antibody
Detection of Human Serpin A1/ alpha1‑Antitrypsin by Western Blot.
Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serpin A1/ alpha1‑Antitrypsin in HepG2 Human Cell Line.
Serpin A1/a1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Serpin A1/ alpha1‑Antitrypsin by Simple WesternTM.
Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha1‑Antitrypsin at approximately 62 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Serpin A1/ alpha1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Applications for Human Serpin A1/ alpha1-Antitrypsin Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A1/ alpha1-Antitrypsin (Catalog # 1268-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A1/ alpha1-Antitrypsin (Catalog # 1268-PI), see our available Western blot detection antibodies
Knockout Validated
Serpin A1 is specifically detected in HepG2 human hepatocellular carcinoma parental cell line but is not detectable in Serpin A1 knockout HepG2 cell line.
Simple Western
10 µg/mL
Sample: Human plasma and human kidney tissue
Sample: Human plasma and human kidney tissue
Western Blot
0.1 µg/mL
Sample: Human plasma, human lung tissue, and human kidney tissue
Sample: Human plasma, human lung tissue, and human kidney tissue
Reviewed Applications
Read 2 reviews rated 4 using AF1268 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A1/alpha 1-Antitrypsin
Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe serpin A1 deficiency (3).
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Barbour, K.W. et al. (2002) Genomics 80:515.
- Lomas, D.A. et al. (2002) Biochem. Soc. Trans. 30:89.
Alternate Names
A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor
Entrez Gene IDs
5265 (Human)
Gene Symbol
SERPINA1
UniProt
Additional Serpin A1/alpha 1-Antitrypsin Products
Product Documents for Human Serpin A1/ alpha1-Antitrypsin Antibody
Product Specific Notices for Human Serpin A1/ alpha1-Antitrypsin Antibody
For research use only
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