Human Serpin A4/Kallistatin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1669
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 209930
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A4/Kallistatin
Leu22-Pro427
Accession # AAA59454
Leu22-Pro427
Accession # AAA59454
Specificity
Detects human Serpin A4/Kallistatin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Serpin A1, A3, A5, C1, F2, or recombinant mouse Serpin D1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Serpin A4/Kallistatin Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI)
Sample: Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A4/Kallistatin
Serpin A4, also called Kallistatin, Kallikrein inhibitor and protease inhibitor 4, is a member of the Serpin superfamily of the serine protease inhibitors (1). Known also as kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth (2, 3).
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Chen, V.C. et al. (2000) J. Biol. Chem. 275:40371.
- Miao, R.Q. et al. (2002) Blood 100:3245.
Alternate Names
Kallistatin
Entrez Gene IDs
5267 (Human)
Gene Symbol
SERPINA4
UniProt
Additional Serpin A4/Kallistatin Products
Product Documents for Human Serpin A4/Kallistatin Antibody
Product Specific Notices for Human Serpin A4/Kallistatin Antibody
For research use only
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