Human Serpin A4/Kallistatin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1669
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A4/Kallistatin
Gly20-Pro427 (predicted)
Accession # AAA59454
Gly20-Pro427 (predicted)
Accession # AAA59454
Specificity
Detects human Serpin A4/Kallistatin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A5, rhSerpin C1, recombinant mouse Serpin D1, and rhSerpin F2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Serpin A4/Kallistatin Antibody
Detection of Human Serpin A4/Kallistatin by Western Blot.
Western blot shows lysates of human liver tissue, human kidney tissue, and human pancreas tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Serpin A4/Kallistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1669) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Serpin A4/Kallistatin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Serpin A4/Kallistatin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI), see our available Western blot detection antibodies
Western Blot
0.25 µg/mL
Sample: Human liver tissue, human kidney tissue, and human pancreas tissue
Sample: Human liver tissue, human kidney tissue, and human pancreas tissue
Neutralization
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A4/Kallistatin
Serpin A4 is a member of the Serpin superfamily of the serine protease inhibitors (1). Known also as Kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth (2, 3).
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Chen, V.C. et al. (2000) J. Biol. Chem. 275:40371.
- Miao, R.Q. et al. (2002) Blood 100:3245.
Alternate Names
Kallistatin
Entrez Gene IDs
5267 (Human)
Gene Symbol
SERPINA4
UniProt
Additional Serpin A4/Kallistatin Products
Product Documents for Human Serpin A4/Kallistatin Antibody
Product Specific Notices for Human Serpin A4/Kallistatin Antibody
For research use only
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