Human Serpin A8/Angiotensinogen Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3156
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A8
Asp34-Ala485
Accession # ABM85122
Asp34-Ala485
Accession # ABM85122
Specificity
Detects human Serpin A8 in Western blots. In this format, less than 1% cross-reactivity with recombinant human Serpin A1, A4, A5, F2, G1, I1, and I2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Serpin A8/Angiotensinogen Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Serpin A8/Angiotensinogen
Sample: Recombinant Human Serpin A8/Angiotensinogen
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A8/Angiotensinogen
Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II. Ang II induces vascoconstriction and aldosterone release. Mature human angiotensinogen is 452 aa in length (aa 34-485). It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41). Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. Over aa 34-485, human serpin A8 shares 61% aa identity with mouse serpin A8.
Alternate Names
AGT, Angiotensin II, Angiotensinogen, Aogen, PAT
Gene Symbol
AGT
UniProt
Additional Serpin A8/Angiotensinogen Products
Product Documents for Human Serpin A8/Angiotensinogen Biotinylated Antibody
Product Specific Notices for Human Serpin A8/Angiotensinogen Biotinylated Antibody
For research use only
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