Human Serpin B2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8550
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 930109
Product Summary for Human Serpin B2 Antibody
Immunogen
E. coli-derived recombinant human Serpin B2
Glu2-Ala65, Ala99-Pro415
Accession # P05120
Glu2-Ala65, Ala99-Pro415
Accession # P05120
Specificity
Detects human Serpin B2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Serpin B2 Antibody
Detection of Human Serpin B2 by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Serpin B2 in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line either untreated (upper panel) or treated with PMA and Calcium Ionomycin for 2 days (lower panel) was stained with Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Serpin B2 in U937 Human Cell Line.
Serpin B2 was detected in immersion fixed U937 human histiocytic lymphoma cell line stimulated with PMA using Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Serpin B2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line treated with PMA and Calcium Ionomycin
Sample: U937 human histiocytic lymphoma cell line treated with PMA and Calcium Ionomycin
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line stimulated with PMA
Sample: Immersion fixed U937 human histiocytic lymphoma cell line stimulated with PMA
Knockout Validated
Serpin B2 is specifically detected in K562 human chronic myelogenous leukemia parental cell line but is not detectable in Serpin B2 knockout K562 cell line.
Western Blot
2 µg/mL
Sample: U937 human histiocytic lymphoma cell line treated with PMA and HEK001 human epidermal keratinocyte cell line
Sample: U937 human histiocytic lymphoma cell line treated with PMA and HEK001 human epidermal keratinocyte cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB8550 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin B2
Serpin B2, also known as Plasminogen Activator Inhibitor 2 (PAI-2), is a 415 amino acid serine-type endopeptidase inhibitor with high expression in blood, vasculature and placenta. Its expression is upregulated in pregnancy and in activated monocytes/macrophages by a wide range of viral, bacterial and parasitic agents. Serpin B2 is upregulated in monocytes from HIV-1 infected patients, and is thought to modulate Th1/Th2 responses. Serpin B2/PAI-2 colocalizes with CHL1 and Vitronectin and mediates neurite outgrowth during post-natal brain development. Inhibition of uPA by SerpinB2 in tumors is associated with a favorable prognosis.
Alternate Names
PAI2, PLANH2, Urokinase Inhibitor
Gene Symbol
SERPINB2
UniProt
Additional Serpin B2 Products
Product Documents for Human Serpin B2 Antibody
Product Specific Notices for Human Serpin B2 Antibody
For research use only
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