What is the light chain of Human Serum Albumin Antibody, Catalog #s MAB1455 and IC1455, Clone #188835?

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin APC-conjugated Monoclonal Antibody (Catalog # IC1455A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Albumin in THP-1 Human Cell Line by Flow Cytometry

THP-1 human monocytic leukemia cell line (negative control) was stained with Mouse Anti-Human Serum Albumin APC-conjugated Monoclonal Antibody (Catalog # IC1455A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Serum Albumin APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

0.01-0.25 µg/10⁶ cells
Sample: HepG2 human hepatocellular carcinoma cell line and THP-1 human monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Albumin

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Alternate Names

ALB

Entrez Gene IDs

213 (Human); 11657 (Mouse); 24186 (Rat)

Gene Symbol

ALB

Additional Albumin Products

Product Documents for Human Serum Albumin APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Serum Albumin APC-conjugated Antibody

For research use only

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Flow Cytometry

Human Serum Albumin APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC1455A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Albumin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Serum Albumin APC-conjugated Antibody

Detection of Albumin in HepG2 Human Cell Line by Flow Cytometry.