Human Serum Amyloid A1/A2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB30196
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 865504
Product Specifications
Immunogen
E. coli-derived recombinant human Serum Amyloid A1
Arg19-Tyr122
Accession # P0DJI8
Arg19-Tyr122
Accession # P0DJI8
Specificity
Detects human Serum Amyloid A1 and Serum Amyloid A2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Serum Amyloid A1/A2 Antibody
Human Serum Amyloid A1/A2 ELISA Standard Curve.
Recombinant human Serum Amyloid A1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30194) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30196) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Serum Amyloid A1/A2 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30194).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Serum Amyloid A1 DuoSet ELISA Kit (Catalog # DY3019-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serum Amyloid A1/A2
Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.
Alternate Names
PIG4;SAA;SAA1;SAA2;Serum amyloid A-1 protein;Serum amyloid A-2 protein;TP53I4
UniProt
Additional Serum Amyloid A1/A2 Products
Product Documents for Human Serum Amyloid A1/A2 Antibody
Product Specific Notices for Human Serum Amyloid A1/A2 Antibody
For research use only
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