Human Serum Amyloid A1/A2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB30192
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 902728
Product Specifications
Immunogen
Human SAA1 synthetic peptide
Accession # P0DJI8
Accession # P0DJI8
Specificity
Detects human SAA1/SAA2 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Serum Amyloid A1/A2 Antibody
Detection of Human Serum Amyloid A1/A2 by Western Blot.
Western blot shows lysates of human spleen tissue, human serum, and human plasma. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30192) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Recombinant Human Serum Amyloid A1/A2 by Western Blot.
Western blot shows 5 ng of Recombinant Human SAA1 and Recombinant Human SAA2. PVDF Membrane was probed with 0.2 µg/mL of Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30192) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Serum Amyloid A1/A2 Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: Human spleen tissue, human serum, human plasma, recombinant human SAA1, and recombinant human SAA2
Sample: Human spleen tissue, human serum, human plasma, recombinant human SAA1, and recombinant human SAA2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serum Amyloid A1/A2
Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.
Alternate Names
PIG4;SAA;SAA1;SAA2;Serum amyloid A-1 protein;Serum amyloid A-2 protein;TP53I4
UniProt
Additional Serum Amyloid A1/A2 Products
Product Documents for Human Serum Amyloid A1/A2 Antibody
Product Specific Notices for Human Serum Amyloid A1/A2 Antibody
For research use only
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