Human Serum Amyloid A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3019
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 370820
Product Specifications
Immunogen
E. coli-derived recombinant human SAA1
Arg19-Tyr122
Accession # P02735
Arg19-Tyr122
Accession # P02735
Specificity
Detects human Serum Amyloid A1 (SAA1) in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant mouse SAA1 and no cross-reactivity with recombinant human (rh) SAA4 is observed. In direct ELISAs, no cross-reactivity with rhSAA2 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Applications for Human Serum Amyloid A1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Serum Amyloid A1
Sample: Recombinant Human Serum Amyloid A1
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serum Amyloid A1
Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.
Alternate Names
PIG4, SAA1
Gene Symbol
SAA1
UniProt
Additional Serum Amyloid A1 Products
Product Documents for Human Serum Amyloid A1 Antibody
Product Specific Notices for Human Serum Amyloid A1 Antibody
For research use only
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