Human SHANK2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7035

R&D Systems, part of Bio-Techne
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MAB7035-SP
MAB7035
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 711924

View all SHANK2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SHANK2
Lys849-Thr1029
Accession # Q9UPX8

Specificity

Detects human SHANK2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SHANK2 Antibody

SHANK2 antibody in Human Brain by Immunohistochemistry (IHC-P).

SHANK2 in Human Brain.

SHANK2 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human SHANK2 Monoclonal Antibody (Catalog # MAB7035) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SHANK2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHANK2

SHANK2 (SH3 and multiple ANKyrin repeat domain protein 2; also CortBP1 and ProSAP1) is a 180-200 kDa member of the Shank family of proteins.  It is expressed in neurons and various epithelial cells, and serves as a multidomain scaffold for cell membrane proteins. For example, in the post-synaptic density of neurons, it provides support for the interaction between glutamate receptors and the actin cytoskeleton. In epithelia, SHANK2 regulates the trafficking and activity of Na/H Exchanger 3. Human SHANK2 is 1470 amino acids (aa) in length. It contains an N-terminal SH3 domain (aa 147-206), a PDZ domain (aa 247-341), a Pro-rich region (aa 509-535), a potential O-linked glycosylation site (aa 948-954) and a C-terminal SAM domain (aa 1407-1470). There are seven potential Ser/Thr phosphorylation sites. Multiple splice forms exist. A 220-240 kDa ankyrin repeat-containing isoform (SHANK2-E) possesses a 391 aa substitution for aa 1-12, and a 165 kDa isoform (potentially known as CortBP1) shows a 28 aa substitution for aa 1-238 coupled with a deletion of aa 383-390. Another potential isoform shows the previously noted 28 aa substitution for aa 1-238 coupled to a 19 aa substitution for aa 472-1470. Over aa 849-1029, human SHANK2 shares 80% aa sequence identity with mouse SHANK2.

Long Name

SH3 and Multiple Ankyrin Repeat Domains Protein 2

Alternate Names

AUTS17, CORTBP1, CTTNBP1, ProSAP1, SPANK-3

Entrez Gene IDs

22941 (Human); 210274 (Mouse)

Gene Symbol

SHANK2

UniProt

Q9UPX8 (Human)

Additional SHANK2 Products

Product Documents for Human SHANK2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human SHANK2 Antibody

For research use only

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