Human SHARPIN Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8100

R&D Systems, part of Bio-Techne
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MAB8100
MAB8100-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 881816

View all SHARPIN Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SHARPIN
Ala27-Ala182
Accession # Q9H0F6

Specificity

Detects human SHARPIN in ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human SHARPIN Antibody

Detection of Human SHARPIN antibody by Western Blot.

Detection of Human SHARPIN by Western Blot.

Western blot shows lysates of U2OS human osteosarcoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SHARPIN Monoclonal Antibody (Catalog # MAB8100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SHARPIN at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SHARPIN Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: U2OS human osteosarcoma cell line and THP‑1 human acute monocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHARPIN

SHARPIN (Shank-associated RH Domain-interacting Protein), also called SIPL1 (Shank Interacting Protein-like 1) is an essential component of the cytosolic LUBAC ubiquitin conjugation complex. It conjugates linear ubiquitin chains to IKK gamma and activates NFk beta, thus regulating immune and inflammatory responses. SHARPIN is widely expressed, with upregulated expression in multiple tumor types. Human SHARPIN is a 387 amino acid (aa) protein containing self-association, SHANK1 interaction, and zinc finger domains. A 326 aa isoform diverges after aa 308 and lacks the zinc finger domain. Within aa 27-182, human SHARPIN shares 67% and 66% aa sequence identity with mouse and rat SHARPIN, respectively.

Long Name

SHANK-associated RH Domain Interactor

Alternate Names

SIPL1

Entrez Gene IDs

81858 (Human); 106025 (Mouse); 81859 (Rat)

Gene Symbol

SHARPIN

UniProt

Q9H0F6

Additional SHARPIN Products

Product Documents for Human SHARPIN Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SHARPIN Antibody

For research use only

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