Human SHBG Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB26562
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 336729
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SHBG
Leu30-His402
Accession # P04278
Leu30-His402
Accession # P04278
Specificity
Detects human SHBG in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SHBG Antibody
Human SHBG ELISA Standard Curve.
Recombinant human SHBG protein was serially diluted 2-fold and captured by Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB26561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB26562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human SHBG Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB26561).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human SHBG DuoSet ELISA Kit (Catalog # DY2656) for convenient development of a sandwich ELISA or the Human SHBG Quantikine ELISA Kit (Catalog # DSHBG0B) for a complete optimized ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SHBG
Human Sex Hormone Binding Globulin (SHBG), also known as ABP or androgen binding protein, is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 aa long laminin a chain G-like domains. Each SHBG monomer is a 47‑53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.
Long Name
Sex Hormone-binding Globulin
Alternate Names
ABP
Entrez Gene IDs
6462 (Human)
Gene Symbol
SHBG
UniProt
Additional SHBG Products
Product Documents for Human SHBG Antibody
Product Specific Notices for Human SHBG Antibody
For research use only
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