Human SHBG Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2656
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SHBG
Leu30-His402
Accession # P04278
Leu30-His402
Accession # P04278
Specificity
Detects human SHBG in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SHBG Biotinylated Antibody
SHBG in Human Liver.
SHBG was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human SHBG Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2656) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human SHBG Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Western Blot
0.1 µg/mL
Sample: Recombinant Human SHBG
Sample: Recombinant Human SHBG
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SHBG
Human SHBG (also known as ABP or androgen-binding protein) is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 amino acid (aa) long laminin alpha chain G-like domains. Each SHBG monomer is a 47-53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.
Long Name
Sex Hormone-binding Globulin
Alternate Names
ABP
Entrez Gene IDs
6462 (Human)
Gene Symbol
SHBG
UniProt
Additional SHBG Products
Product Documents for Human SHBG Biotinylated Antibody
Product Specific Notices for Human SHBG Biotinylated Antibody
For research use only
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