Human SIGIRR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB990

R&D Systems, part of Bio-Techne
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MAB990
MAB990-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 162201

View all SIGIRR Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SIGIRR
Met1-His118
Accession # Q6IA17

Specificity

Detects human SIGIRR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) IL‑1 RI, rhIL‑1 R3, rhIL-1 R8, rhIL-1 RII, rhIL-1 R6, rhIL-1 R4, rhIL-18, rhIL-1 R7, rhIL-9 R9, or recombinant mouse SIGIRR.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human SIGIRR Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human SIGIRR Fc Chimera (Catalog # 990-SG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIGIRR

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL1RAPL1), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Five of the IL-1 R family members (1, 2, 4, 5, and 6) are clustered and localized to chromosome 2. SIGIRR (single Ig domain containing IL-1 receptor-related molecule) is a subtype of the IL-1 R family that differs from the other nine members by having only one Ig domain in its extracellular region. The sequence of human SIGIRR predicts a 410 amino acid (aa) residue transmembrane glycoprotein that lacks signal peptide and contains a 118 aa single Ig extracellular domain, a transmembrane region and a 268 aa cytoplasmic tail with a TIR domain. The cytoplasmic tail of SIGIRR contains a C-terminal extention beyond the TIR domain which is also found in IL1RAPL1, IL-1 R9, and Toll-like receptor family members but absent in other IL-1 receptor family members. SIGIRR is widely expressed and is present in all cells and tissues examined. Mouse and human SIGIRR share 82% amino acid sequence identity. The ligand and signaling mechanism for SIGIRR has not been identified.

References

  1. Thomassen, E. et al. (1999) Cytokine 11:389.

Long Name

Single lg IL-1 Related Protein

Alternate Names

IL-1R8, TIR8

Entrez Gene IDs

59307 (Human); 24058 (Mouse)

Gene Symbol

SIGIRR

UniProt

Q6IA17

Additional SIGIRR Products

Product Documents for Human SIGIRR Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SIGIRR Antibody

For research use only

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