Human Siglec-16 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB68191
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 706045
Product Specifications
Immunogen
Human Siglec-16 peptide
Accession # A6NMB1
Accession # A6NMB1
Specificity
Detects human Siglec-16 in direct ELISAs. In flow cytometry, detects human Siglec-16, but not human Siglec-11, in transfected HEK293 cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Siglec-16 Antibody
Detection of Siglec-16 in HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP by Flow Cytometry
HEK293 human embryonic kidney cell line transfected with (A) human Siglec-16 or (B) human Siglec-11, and eGFP was stained with Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68191) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041) .View our protocol for Staining Membrane-associated Proteins.
Detection of Siglec-16 in CHO cells transfected with human Siglec-16 or irrelevant transfectant cells by Flow Cytometry.
CHO cells transfected with human Siglec-16 (filled histogram) or irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68191), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.Applications for Human Siglec-16 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP and CHO cells transfected with human Siglec-16 or irrelevant transfectant cells
Sample: HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP and CHO cells transfected with human Siglec-16 or irrelevant transfectant cells
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: CHO cells transfected with human Siglec-16 or irrelevant transfectant
Sample: CHO cells transfected with human Siglec-16 or irrelevant transfectant
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Siglec-16
Siglec-16 (Sialic acid-binding Ig-like lectin 16) is a 58-60 kDa member of the CD33-related SIGLEC family of proteins. It is expressed on macrophages and microglia, and based on Siglec-11, likely binds to an alpha2,8-linked sialic acid motif. Although Siglec-16 is assumed to have arisen from a Siglec-11 gene duplication and conversion, it is not an inhibitory receptor but an activating one, and possesses a transmembrane (TM)
Long Name
Sialic Acid Binding Ig-like Lectin 16
Alternate Names
Siglec16, SIGLECP16
Entrez Gene IDs
400709 (Human)
Gene Symbol
SIGLEC16
UniProt
Additional Siglec-16 Products
Product Documents for Human Siglec-16 Antibody
Product Specific Notices for Human Siglec-16 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...