Human Sigma-1 R Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1076
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1319A
Product Specifications
Immunogen
Synthetic peptide containing human Sigma-1 R
Accession # Q99720
Accession # Q99720
Specificity
Detects human Sigma-1 R in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Sigma-1 R Antibody
Sigma-1 R in HeLa Human Cell Line.
Sigma-1 R was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Sigma-1 R Monoclonal Antibody (Catalog # MAB1076) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Sigma-1 R Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sigma-1 R
Sigma non-opioid intracellular receptor 1 (SIGMAR1, OPRS1 or Sigma1 receptor) is a 223 aminoacids (aa), 25KDa chaperone protein at the endoplasmic reticulum (ER) that modulates calcium signaling through the IP3 receptor. Sigmar1 functions as a lipid transporter and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. In Human, at least 4 other isoforms have been found, ranging from 12-22 KDa.
Long Name
Sigma Non-opioid Intracellular Receptor 1
Alternate Names
ALS16, OPRS1, SIG-1R, Sigma-1R, Sigma1R, SIGMAR1, SRBP
Gene Symbol
SIGMAR1
UniProt
Additional Sigma-1 R Products
Product Documents for Human Sigma-1 R Antibody
Product Specific Notices for Human Sigma-1 R Antibody
For research use only
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