HEK293 cells transfected with Human SIRP delta (filled histogam) or irrelevant protein (open histogram) were stained with Rabbit Anti-Human SIRP delta Monoclonal Antibody (Catalog # MAB10138) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

SIRP delta in THP‑1 Human Cell Line.

SIRP delta was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (left panel; positive staining) and MCF-7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human SIRP delta Monoclonal Antibody (Catalog # MAB10138) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human SIRP delta Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human cell line transfected with Human SIRP delta

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP delta

SIRPD (Signal Regulatory Protein Delta), also know as Protein Tyrosine Phosphatase non-Receptor Type Substrate 1-l Like 2 (PTPNS1L2), is member of the signal regulatory proteins (SIRPS) family (1). SIRPD contains a 168 amino acid Ig-like domain that is characteristic of other SIRP family members (1). Unlike other members of SIRPS family, SIRPD lacks the transmemberane region, and is secreted (2). Murine homologs of SIRPD are not characterized. Expression sequence tag analysis suggests that SIRPD may be expressed in sperm cells and respiratory tissue (2). Using BioPlex 2.0 (Biophysical Interactions of ORFeome-derived complexes) high-throughput affinity purification–mass spectrometry (AP–MS) analysis to identify probable protein–protein interactions, several candidate SIRPD interactions were found including DIRAS2 (3). In-house testing indicates SIRPD can interact with DIRAS2.

References

Van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
Van Beek, E.M. et al. (2005) J Immunol. 175:7781.
Huttlin, E.L. et al. (2017) Nature 545:505.

Long Name

Signal-regulatory Protein delta

Alternate Names

dJ576H24.4, PTPNS1L2, SIRP-Delta, SIRPD

Entrez Gene IDs

128646 (Human)

Gene Symbol

SIRPD

UniProt

Q9H106

Additional SIRP delta Products

Product Documents for Human SIRP delta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SIRP delta Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human SIRP delta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10138

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SIRP delta Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SIRP delta Antibody

Detection of SIRP delta on HEK293 Human Cell Line transfected with Human SIRP delta by Flow Cytometry.