Human peripheral blood granulocytes were stained with Mouse Anti-Human SIRP-beta Monoclonal Antibody (Catalog # MAB20961, filled histogram) or Mouse IgG1 Isotype Control Antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

SIRP beta 1/CD172b in U937 human histiocytic lymphoma cell line.

SIRP beta1/CD172b was detected in immersion fixed U937 human histiocytic lymphoma cell line (left panel; positive staining) and Daudi human Burkitt's lymphoma cell line (right panel; negative staining) using Mouse Anti-Human SIRP beta1/CD172b Monoclonal Antibody (Catalog # MAB20961) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human SIRP beta1/CD172b Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human Peripheral Blood Granulocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP beta 1/CD172b

SIRP beta1 is a type I transmembrane protein belonging to the SIRP family within the Ig superfamily. Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. Unlike SIRP alpha that has cytoplasmic ITIM domains, SIRP beta1 possesses positively charged residues that allow association with ITAM motif containing adaptor molecules. SIRP beta1 is expressed on cells of monocyte, macrophage or dendritic lineages.

Long Name

Signal-regulatory Protein beta 1

Alternate Names

CD172b, SIRPB1

Entrez Gene IDs

10326 (Human); 102139733 (Cynomolgus Monkey)

Gene Symbol

SIRPB1

UniProt

O00241.4

Additional SIRP beta 1/CD172b Products

Product Documents for Human SIRP beta1/CD172b Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human SIRP beta1/CD172b Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human SIRP beta1/CD172b Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB20961

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SIRP beta 1/CD172b Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SIRP beta1/CD172b Antibody

Detection of SIRP-beta in Human Peripheral Blood Granulocytes by Flow Cytometry.