HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Slug PE-conjugated Monoclonal Antibody (Catalog # IC7408P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Slug PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Slug

SLUG, also known as SNAI2, is a zinc-finger transcription factor. SLUG is a transcriptional repressor which may suppress E-cadherin transcription and be involved in an epithelial to mesenchymal transition (EMT).

Alternate Names

SLUGH1, SNAI2, SNAIL2, WS2D

Entrez Gene IDs

6591 (Human); 20583 (Mouse)

Gene Symbol

SNAI2

UniProt

O43623 (Human)

Additional Slug Products

Product Documents for Human Slug PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Slug PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Slug PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC7408P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Slug Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Slug PE-conjugated Antibody

Detection of Slug in HeLa Human Cell Line by Flow Cytometry.