Human SLURP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4479
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SLURP1
Ala22-Leu103
Accession # P55000
Ala22-Leu103
Accession # P55000
Specificity
Detects human SLURP1 in direct ELISAs. In direct ELISAs, less than 3% cross-reactivity with recombinant mouse SLURP1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human SLURP1 Antibody
SLURP1 in Human Skin.
SLURP1 was detected in immersion fixed paraffin-embedded sections of human skin using Sheep Anti-Human SLURP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4479) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human SLURP1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin
Sample: Immersion fixed paraffin-embedded sections of human skin
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SLURP1
SLURP1 (Secreted Ly6/uPAR-related protein 1; also ARS Component B and ANUP) is a 9-10 kDa secreted member of the Ly6/uPAR Superfamily of proteins. It is expressed by restricted cell types, including sensory neurons, ciliated bronchial epithelium, T and B cells, and stratified squamous epithelium of the skin, vagina and esophagus. SLURP1 binds to the alpha7 nicotinic acetylcholine receptor, which suggests it regulates Ca and Na transport. This translates into reduced TNF alpha release by macrophages, and activation of select cellular caspases. Mature human SLURP1 is 82 amino acids (aa) in length (aa 22-103). It contains one UPAR/Ly6 domain (aa 24‑73), and forms noncovalent homodimers. Mature human SLURP1 shares 67% aa identity with mouse SLURP1.
Long Name
Secreted Ly-6/uPAR-related Protein 1
Alternate Names
ARS Component B
Gene Symbol
SLURP1
UniProt
Additional SLURP1 Products
Product Documents for Human SLURP1 Antibody
Product Specific Notices for Human SLURP1 Antibody
For research use only
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